Revolutionize your molecular biology workflow with the BioExtract™ All-in-One Nucleic Acid Isolation & qPCR/RT-qPCR Detection Kit. This comprehensive solution seamlessly combines high-efficiency purification of both RNA and DNA from a wide range of sample types with sensitive and specific detection capabilities using real-time PCR (qPCR) and reverse transcription real-time PCR (RT-qPCR).
Designed for convenience, speed, and reliability, the BioExtract™ Kit minimizes hands-on time, reduces the risk of cross-contamination, and delivers highly pure nucleic acids suitable for the most demanding downstream applications, including direct integration into real-time detection without transferring to separate kits.
Key Features & Benefits
Integrated Workflow: Perform both RNA/DNA extraction and real-time detection (qPCR/RT-qPCR) using a single, optimized kit. No need for separate extraction and detection reagents, saving time and reducing protocol complexity.
Broad Sample Compatibility: Efficiently extract high-quality RNA and DNA from diverse sample types, including:
Blood (whole blood, plasma, serum)
Tissue (fresh, frozen, FFPE)
Saliva, Urine, CSF
Swabs (nasopharyngeal, buccal, environmental)
Cells (mammalian, bacterial, yeast)
Cell-free samples
Superior Nucleic Acid Purity & Yield: Proprietary lysis and binding buffers, combined with advanced spin-column or magnetic bead technology, ensure isolation of highly pure RNA and DNA free from inhibitors, proteins, and other contaminants.
Yield: Up to 20 µg total nucleic acid from 200 µL whole blood, depending on sample type and cellularity.
Purity: A260/280 ratios typically 1.8-2.0, A260/230 ratios typically >2.0, ensuring optimal performance in downstream enzymatic reactions.
Rapid & Efficient Protocol:
Extraction: ~30-45 minutes for manual spin-column/magnetic bead protocol.
Detection: ~60-90 minutes for complete qPCR/RT-qPCR run.
High Sensitivity & Specificity: Optimized master mixes (Taq Polymerase, Reverse Transcriptase) and reaction conditions enable robust detection of even low-abundance targets, providing accurate and reproducible results.
Versatile Detection: Compatible with standard 96-well and 384-well real-time PCR instruments and various probe-based (TaqMan™, Molecular Beacons) and dye-based (SYBR™ Green) detection chemistries.
Integrated Controls: Includes all necessary reagents for both extraction and detection, along with internal control options to monitor extraction efficiency and inhibiton.
Reduced Contamination Risk: Streamlined protocol and minimal tube transfers reduce potential for cross-contamination, crucial for sensitive applications.
Automation-Friendly (Magnetic Bead version): The magnetic bead-based kit is readily adaptable to automated liquid handling systems for high-throughput processing.
Applications
The BioExtract™ All-in-One Kit is ideal for a wide range of molecular biology applications, including:
Pathogen Detection: Viral, bacterial, and fungal diagnostics in clinical, veterinary, and food safety settings.
Gene Expression Analysis: Quantification of mRNA levels from various sample sources.
Genetic Variation Analysis: SNP genotyping, mutation detection, and genetic disease screening.
Epidemiology & Surveillance: Monitoring disease outbreaks and population genetics.
Environmental Monitoring: Detecting specific organisms or genetic material in environmental samples.
Research & Development: A foundational tool for numerous molecular biology investigations.
Principle
The BioExtract™ Kit employs a multi-step process for efficient and reliable results:
Lysis: Samples are homogenized and lysed using a potent lysis buffer containing chaotropic salts and detergents. This simultaneously denatures proteins and inactivates nucleases, preserving nucleic acid integrity.
Binding: Nucleic acids (both RNA and DNA) are selectively bound to a silica-based membrane (spin-column format) or magnetic beads (magnetic bead format) under optimized conditions, while contaminants are washed away.
Washing: Multiple wash steps effectively remove residual inhibitors, salts, and proteins, ensuring high purity.
Elution: Pure, high-quality RNA and DNA are eluted in a low-salt elution buffer or nuclease-free water, ready for immediate use.
Detection: The purified nucleic acid is directly added to a pre-formulated master mix containing a highly sensitive reverse transcriptase (for RNA), a hot-start DNA polymerase, dNTPs, optimized reaction buffers, and user-defined primers/probes for real-time PCR detection.
Kit Components
Each BioExtract™ All-in-One Kit contains all reagents necessary for both nucleic acid extraction and real-time PCR detection:
