Description

Here is a detailed product description for the Diaclone France IL-6 ELISA Kit in Markdown format:

Diaclone France IL-6 ELISA Kit

Quantify Human Interleukin-6 with Precision and Reliability

Elevate your research into inflammation, immunity, and disease pathogenesis with the Diaclone France IL-6 ELISA Kit. Specifically designed for the accurate and quantitative detection of human Interleukin-6 (IL-6) in various biological samples, this robust assay provides the sensitivity and specificity essential for cutting-edge scientific discovery.

Diaclone, a trusted name in immunology research, delivers a high-quality, ready-to-use ELISA kit that streamlines your workflow and provides consistent, reproducible results, making it an indispensable tool for researchers worldwide.

Key Features & Benefits

• Exceptional Sensitivity: Detects even low physiological concentrations of human IL-6, allowing for comprehensive analysis of subtle biological changes.
• High Specificity: Engineered to minimize cross-reactivity with other related cytokines, ensuring that you are precisely measuring IL-6 without interference.
• Wide Dynamic Range: Provides a broad measurement range, accommodating various IL-6 concentrations encountered in diverse experimental conditions and sample types.
• Reliable & Reproducible Results: Manufactured under stringent quality control standards, guaranteeing consistent performance across experiments and between lots.
• User-Friendly Protocol: Features a clear, step-by-step protocol for efficient laboratory workflow and reduced hands-on time.
• Validated for Diverse Samples: Optimized and validated for use with common biological samples including serum, plasma, and cell culture supernatant.
• Complete Kit: Contains all necessary reagents, including pre-coated plates, standards, and detection antibodies, for a full 96-well assay.

Principle of the Assay

The Diaclone Human IL-6 ELISA Kit operates on a sandwich enzyme-linked immunosorbent assay format.

1. A 96-well microplate is pre-coated with a high-affinity monoclonal antibody specific for human IL-6.
2. Standards and samples are added to the wells, allowing any present IL-6 to bind to the immobilized capture antibody.
3. After a washing step, a biotinylated anti-human IL-6 detection antibody is added, forming a sandwich complex.
4. Following another wash to remove unbound detection antibody, Streptavidin-HRP conjugate is added, binding to the biotinylated antibody.
5. Unbound HRP conjugate is washed away, and a Tetramethylbenzidine (TMB) substrate solution is added, which is then catalyzed by the bound HRP to produce a blue color.
6. The reaction is stopped by the addition of an acid solution, turning the color yellow.
7. The intensity of the yellow color is directly proportional to the amount of human IL-6 initially present in the sample and is measured spectrophotometrically at 450 nm with a reference wavelength of 620 nm.

Applications

This highly versatile ELISA kit is an essential tool for researchers investigating:

• Immunology and Inflammation: Monitoring IL-6 as a key mediator in acute and chronic inflammatory responses, autoimmune diseases (e.g., rheumatoid arthritis, lupus), and sepsis.
• Oncology: Studying the role of IL-6 in cancer progression, metastasis, immune evasion, and the tumor microenvironment.
• Infectious Diseases: Analyzing cytokine storms and host immune responses during viral (e.g., COVID-19), bacterial, and parasitic infections.
• Neuroscience: Investigating neuroinflammation and IL-6's involvement in neurological disorders such as Alzheimer's disease, Parkinson's disease, and stroke.
• Cardiovascular Research: Examining IL-6's contribution to atherosclerosis, myocardial infarction, and heart failure.
• Drug Discovery & Development: Evaluating the efficacy of anti-inflammatory, immunomodulatory, and targeted therapies.
• Biomarker Research: Identifying and validating IL-6 as a diagnostic, prognostic, or predictive biomarker in various disease states.

Performance Specifications

• Analyte: Human Interleukin-6 (IL-6)
• Detection Range: Typically 3.125 - 200 pg/mL (Refer to lot-specific Certificate of Analysis for exact range)
• Sensitivity (Lowest Detection Limit): < 1 pg/mL
• Specificity: Highly specific for human IL-6. No significant cross-reactivity observed with other human cytokines (e.g., IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, IFN-γ, TNF-α, GM-CSF).
• Sample Types: Serum, Plasma (EDTA, Heparin, Citrate), Cell Culture Supernatant, and other biological fluids (user validated).
• Assay Time: Approximately 3.5 - 4 hours
• Detection Method: Colorimetric (absorbance at 450 nm with 620 nm reference wavelength)
• Assay Format: 96-well microplate (12 x 8 removable strips)

Kit Components

Each Diaclone France IL-6 ELISA Kit typically includes:

• Pre-coated Microplate: 1 x 96-well (12x8 strip format) microplate coated with anti-human IL-6 capture antibody.
• Human IL-6 Standard: Lyophilized recombinant human IL-6 for standard curve generation.
• Biotinylated Anti-human IL-6 Detection Antibody: Lyophilized.
• Streptavidin-HRP Conjugate: Liquid concentrate.
• TMB Substrate Solution: Ready-to-use.
• Stop Solution: Ready-to-use.
• Wash Buffer Concentrate: 20X concentrate.
• Assay Diluent: Ready-to-use.
• Plate Sealers: For incubation steps.
• Detailed User Manual: Comprehensive instructions for assay execution and data analysis.

Storage & Stability

• Upon Receipt: Store the complete kit at 2-8°C in the dark.
• Shelf Life: Refer to the kit label for the exact expiry date, typically 12 months from the date of manufacture when stored properly.
• Opened Reagents: Individual reagents should be stored as per the instructions in the user manual to maintain optimal performance and stability.

Ordering Information

• Product Name: Diaclone France IL-6 ELISA Kit
• Catalog Number: 860.000.006
• Size: 1 x 96 tests
• Manufacturer: Diaclone SAS, France

Technical Support & Resources

Diaclone is committed to providing exceptional customer support. For detailed protocols, troubleshooting guides, sample preparation advice, or technical assistance, please refer to the comprehensive user manual included with the kit or contact our expert technical support team.

Visit the Diaclone Official Website for the latest product information, scientific publications, and additional resources.